TRYPSIN-BOVINE USP
T-82848

DESCRIPTION:
T82848 is a white powder manufactured from bovine pancreas collected from BSE, TSE free countries currently on the European Unions free countries list. The materail is manufactured to meet or exceed USP standards During the manufacture the Trypsin is subjected to pH of <2 and further purified.

APPLICATIONS:

  • Used extensively in protein sequencing
  • Tissue culture work
  • Tissue dissociation
  • Microbiological media
ANALYSIS SPECIFICATION TYPICAL
ASH (RESIDUE UPON IGNITION) Maximum 2.5% 0.88%
MOISTURE (LOSS ON DRYING) Maximum 5% 2.5 %
AEROBIC COUNT <100 organisms/gram 00.0 organisms/gram
PSEUDOMONUS AERUGINOSA Not Detected Not Detected
E. COLI Not Detected Not Detected
SALMONELLA Not Detected Not Detected
STAPHYLOCOCCUS AUREUS Not Detected Not Detected
ASSOCIATED ACTIVITY Report Chymotrypsin Report Chymotrypsin
TRYPSIN ACTIVITY >2500 USP units/mg 2900 USP units/mg
SOLUBILITY 500,000 USP Trypsin units
In 10 ml water
Clear-buff solution
SOLUBILITY 500,000 USP Trypsin units
In 10 ml saline
Clear-buff solution
STORAGE: 2-8C

CHYMOTRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE of 0.0075 PER MINIUTE AT 25C.
TRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE of 0.003 PER MINIUTE AT 25C.
METHOD OF ASSAY: SCHWERT,G.W. AND TAKENAKA.

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