TRYPSIN-PORCINE
T-77
DESCRIPTION:
Trypsin T-77 is a proteolytic enzyme that is a off white to beige colored powder. T-77 is extracted from USDA certified porcine pancreas glands. It is classified as a Serine type protease.
APPLICATIONS:
- Used extensively in protein sequencing
- Tissue culture work
- Tissue dissociation
- Microbiological media
| ANALYSIS | SPECIFICATION | TYPICAL |
| ASH (RESIDUE UPON IGNITION) | Maximum 2.5% | 0.2% |
| MOISTURE (LOSS ON DRYING) | Maximum 5% | 3.0% |
| STANDARD PLATE COUNT | Maximum 1000 CFU/gram | <1000 CFU/gram |
| YEAST/MOLD | Maximum 10 CFU/gram | 6 CFU/gram |
| ESCHERICHIA COLI | Not Detected in 25 grams | Not Detected |
| PSEUDOMONUS AERUGINOSA | Not Detected in 25 grams | Not Detected |
| SALMONELLA | Not Detected in 25 grams | Not Detected |
| STAPHYLOCOCCUS AUREUS | < 10 CFU/GRAM | Not Detected |
| PARVOVIRUS | Negative | Negative |
| ENDOTOXIN (GEL CLOT) | <10 EU/mg | <1 EU/mg |
| CHYMOTRYPSIN ACTIVITY | MAXIMUM 75 UNITS/ mg | 47 UNITS/mg |
| TRYPSIN ACTIVITY | MAXIMUM 250 UNITS/ mg | MINIMUM 250 UNITS/ mg |
CHYMOTRYPSIN:: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE AT 237 nm
of 0.00075 PER MINIUTE AT 25C.
TRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE AT 253 nm of 0.003 PER MINIUTE AT 25C.
PORCINE PARVOVIRUS: TESTED VIA PCR OR 9CFR METHOD